The Intercellular Adhesin Involved in Biofilm Accumulation of Staphylococcus epidermidis Is a Linear b-1,6-Linked Glucosaminoglycan: Purification and Structural Analysis
نویسندگان
چکیده
DIETRICH MACK,* WERNER FISCHER, ANDREAS KROKOTSCH, KLAUS LEOPOLD, RUDOLF HARTMANN, HEINZ EGGE, AND RAINER LAUFS Institut für Medizinische Mikrobiologie und Immunologie, Universitätskrankenhaus Eppendorf, D-20246 Hamburg, Institut für Biochemie der Medizinischen Fakultät, Universität Erlangen-Nürnberg, D-91054 Erlangen, and Institut für Physiologische Chemie, Universität Bonn, D-53115 Bonn, Federal Republic of Germany
منابع مشابه
The intercellular adhesin involved in biofilm accumulation of Staphylococcus epidermidis is a linear beta-1,6-linked glucosaminoglycan: purification and structural analysis.
The primary attachment to polymer surfaces followed by accumulation in multilayered cell clusters leads to biofilm production of Staphylococcus epidermidis, which is thought to contribute to virulence in biomaterial-related infections. We purified a specific polysaccharide antigen of biofilm-producing S. epidermidis 1457 and RP62A, which was recently shown to have a function in the accumulative...
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Clinical isolates of coagulase-negative staphylococci often elaborate a biofilm involved in adherence to medical devices and resistance to host defenses. The biofilm contains the capsular polysaccharide/adhesin (PS/A), which mediates cell adherence to biomaterials, and another antigen, termed polysaccharide intercellular adhesin (PIA), which is thought to mediate bacterial accumulation into cel...
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Virulence of the nosocomial pathogen Staphylococcus epidermidis is crucially linked to formation of adherent biofilms on artificial surfaces. Biofilm assembly is significantly fostered by production of a bacteria derived extracellular matrix. However, the matrix composition, spatial organization, and relevance of specific molecular interactions for integration of bacterial cells into the multil...
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Products of the intercellular adhesion (ica) operon in Staphylococcus aureus and Staphylococcus epidermidis synthesize a linear beta-1,6-linked glucosaminylglycan. This extracellular polysaccharide mediates bacterial cell-cell adhesion and is required for biofilm formation, which is thought to increase the virulence of both pathogens in association with prosthetic biomedical implants. The envir...
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Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less w...
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